ReoRox G2 Assay®
Introduction
The ReoRox G2® assay is a viscoelastic-based system that measures the viscoelastic properties of whole blood using free oscillation. See Reference 5 for more information on the methodology.
Variables measured by the ReoRox® Assay
| Variable | Comments |
|---|---|
| Coagulation Time 1 [COT1] | The time to the start of clot formation and obtained from the Viscosity curve |
| Coagulation Time 2 [COT2] | The time to complete clot formation and after which elasticity builds up. The COT2 time is equivalent to the CT for the ROTEM and the K time for the TEG. |
| Clot Strength in terms of maximum elasticity [G'max] | Derived from the elasticity curve and is equivalent to the MCF for the ROTEM and MA for the TEG. |
| Slope | Similar to the slope between the R and K times on the TEG and the angle of tangent at 2mm above baseline on the ROTEM. |
| Clot SR | Start of clot lysis |
| Clot T | Completion of clot lysis |
A number of modifications of the methodology are available that allow measurement of various parameters:
Pre-configured ReoRox Kits
| Modification | Interpretation | TEG/ROTEM Devices |
|---|---|---|
| HepScreen1 | Designed to distinguish between the effects of Heparin on coagulation for other disorders of coagulation. Uses Thromboplastin to activate the Extrinsic pathway of coagulation. |
Similar to the Kaolin TEG on the TEG machine and the INTEM on the ROTEM device |
| HepScreen2 | Uses Thromboplastin to activate the Extrinsic pathway of coagulation + Heparin neutralisation | Similar to the HepTEG on the TEG machine [uses a heparin-neutralising cup] and the HEPTEM on the ROTEM device |
| FibScreen1 | Uses Thromboplastin to activate the Extrinsic pathway of coagulation. | Similar to the Rapid TEG on the TEG machine [uses both Kaolin and Tissue Factor activation] and EXTEM on the ROTEM machine. Similar to the Activated Clotting Time [ACT] |
| FibScreen2 | Uses Thromboplastin to activate the Extrinsic pathway of coagulation + inhibition of platelets with Abciximab [a potent platelet GpIIb/IIIa inhibitor]. Inhibition of platelet function allows the measurement of functional fibrinogen levels. | Similar to the FIBTEM on the ROTEM machine |
| ReoTrap | Activation of platelets via PAR-1. Can be used to evaluate patients with defective or abnormal platelet activation. | Similar to the Platelet Mapping Kit on the TEG |
| ReoLyse test | Measurement of fibrinolysis | |
| Hct% | Allows the measurement of the Hct in whole blood | |
| PT and APTT | Can be analysed on whole blood |